Eppendorf BioSpectrometer® fluorescence

Image – BioSpectrometer fluorescence front view
Image – BioSpectrometer fluorescence
Image – BioSpectrometer fluorescence product_002
Image – VIS Cuvette macro
Image – product_004
Image – µCuvette G1.0
Image – Food and Beverage, BioPhotometer, BioSpectrometer
Image – UVette light paths_white
Image – µCuvette G1.0 closed
Image – VIS Cuvette semi-micro
Image – BioSpectrometer fluorescence product_003
Image – BioSpectrometer fluorescence front view
Image – BioSpectrometer fluorescence
Image – BioSpectrometer fluorescence product_002
Image – VIS Cuvette macro
Image – product_004
Image – µCuvette G1.0
Image – Food and Beverage, BioPhotometer, BioSpectrometer
Image – UVette light paths_white
Image – µCuvette G1.0 closed
Image – VIS Cuvette semi-micro
Image – BioSpectrometer fluorescence product_003
Eppendorf BioSpectrometer® fluorescence
Eppendorf BioSpectrometer® fluorescence

Focusing on very small sample amounts?
The integrated fluorescence unit of the BioSpectrometer fluorescence increases the measuring range by a factor of 1,000, for example, to detect DNA. This enables reliable quantification down to a concentration of 1.0 pg/µL. Measurements can be flexibly executed in the UV/Vis or fluorescence range. More information

Order Info
Net Price
$ 11,555.26
(inc gst: $ 12,710.79)
Your Price
$ 11,555.26
(inc gst: $ 12,710.79)
Net Price
$ 12,814.36
(inc gst: $ 14,095.80)
Your Price
$ 12,814.36
(inc gst: $ 14,095.80)

Product Information

The Eppendorf BioSpectrometer fluorescence is an expansion of the Eppendorf BioSpectrometer basic and features an integrated fluorescence module. UV/Vis assays, fluorescence assays and semi-micro/micro-volume measurements can all be carried out on a single instrument without the need for external control (PC/Laptop). Eppendorf BioSpectrometer fluorescence can be used to increase the measuring range for biomolecules (nucleic acids and proteins) by a factor of 1,000, compared to UV measurements. Pre-programmed (modified) methods for Qubit DNA quantification kits (SS, HS, BR) expand the flexibility and functionality of the instrument. Additionally, the use of the Eppendorf µCuvette G1.0 for fluorescence assays (5 µL loading volume) allows for a vast reduction in reagent usage.

Standard data


Standard values are to be defined and measured by the instrument.

Eppendorf BioSpectrometer® fluorescence

Set the standard curve


Based on the standard data, the instrument calculates the standard curve. This includes the linear regression value as well as the coefficient of determination.

Eppendorf BioSpectrometer® fluorescence

Sample results


The measured sample value is recalculated based on the standard curve. Finally, you get a concentration value for your sample.

Eppendorf BioSpectrometer® fluorescence

Annoyed by uncomfortable handling?


Ergonomics is far beyond the »ergonomically designed chair«. Eppendorf started to optimize the laboratory devices regarding ergonomics already in the early 1970s.
In 2003, we started the PhysioCare Concept, focusing on ergonomic liquid handling devices like our pipettes.
Nowadays, the Eppendorf PhysioCare Concept is broaden up to include further laboratory products as a holistic solution to harmonize the workflow in your laboratory with your health and well-being, e.g. for the Eppendorf BioSpectrometer:
› Guided software for easy handling
› Clear indications at cuvette shaft for convenient handling
› Direct keys for convenient handling
› Small footprint to enable position in direct arm reach at your bench
› Standardized »user-interface concept« for intuitive handling of devices

Further information about the Eppendorf PhysioCare Concept:
www.eppendorf.com/physiocare/

Eppendorf BioSpectrometer® fluorescence

Struggling with paper-based documentation?


The eLABJournal Electronic Lab Notebook offers an intuitive and flexible solution to document research and track research data like BioSpectrometer–based values. Improve efficiency when documenting, organizing, searching, and archiving collected data. With the free eLABJournal add-ons, you can extend the functionality of eLABJournal to a fully integrated Laboratory Information Management System.

Start a free 30-day trial!
Visit www.eLABJournal.com

Eppendorf BioSpectrometer® fluorescence

Features

  • All Eppendorf BioSpectrometer basic options
  • Preprogrammed and freely programmable fluorescence methods
  • Preprogrammed (modified) methods for Qubit DNA quantification kits (SS, HS, BR)
  • Fluorescence measurement concentration of dsDNA down to 1 pg/µL, using Pico Green®
  • Fluorescence measurement concentration of BSA down to 0.01 ng/µL
  • Fluorescence excitation wavelength 470 nm, emission wavelengths 520 nm and 560 nm
  • Fluorescence intensity across a range of 0.5 nM to 2,000 nM fluorescein
  • Single consumable (UVette) for UV/Vis and fluorescence assays
  • Data transfer via USB interface, Ethernet or via e-mail and direct printout of results
  • Data output as *.PNG screenshots, Microsoft® Excel®, or secure PDF files to a standard USB flash drive or via email

Applications

  • Ideal for NGS applications (micro-volume UV/Vis and fluorescence assays in a single instrument)
  • dsDNA quantification down to 1 pg/µL with common kits, e.g., Pico Green®
  • ssDNA quantification down to 10 pg/µL
  • RNA quantification down to 20 pg/µL
  • Oligo quantification down to 10 pg/µL
  • Protein (BSA) quantification down to 0.01 ng/µL
  • Quantification of GFP-binding proteins
  • Growth measurement of GFP-producing bacteria
Eppendorf BioSpectrometer® fluorescence
Video: Eppendorf BioSpectrometer® fluorescence – Video – University of East London
Eppendorf BioSpectrometer® fluorescence
Video: Eppendorf BioSpectrometer® fluorescence – Video – Eppendorf BioSpectrometer fluorescence

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